Identification and Quantification of Phenolic Compounds from Different Iranian Varieties Pomegranate Peel

Pomegranate (Punica granatum) is one of the most consumed fruits in different parts of the world, including Iran, India, and Turkey. Peels of pomegranate have been reported as a rich source compound such as ellagitannins with antioxidant, anti-inflammatory, anti-hepatotoxicity, anti-atherosclerotic capacity, and antimicrobial properties [۱,۲]. In this study, the pomegranate samples were collected in September ۲۰۱۸ from mature bearing trees growing in the Agricultural Research Center of Yazd, Iran. The peels of different samples separated from arils and dried on the shadow at room temperature. The peels extracted using ethanol as solvent and the samples qualitatively and quantitatively analyzed by HPLC-DAD–ESI/MS. In total, ۹ compounds were detected, among which ellagic acid (۱.۶۰-۱۳.۳۹ mg/g DW), ellagic acid derivatives (۰.۱۴-۲۰.۸۰ mg/g DW), punicalagin (۱۳.۸۷-۴۸.۶۹ mg/g DW) and, punicalagin derivatives (۱.۷۴-۱۶.۹۲ mg/g DW) were identified based on their UV spectra and fragmentation patterns in the mass spectra in different genotypes. Also, the antioxidant activity, total phenolic, and total flavonoid content were determined. The results showed high antioxidant activity (۳.۸۱-۱۴.۶۲ μg/ml), the polyphenol content (۶۶.۳۸-۱۸۱.۴۱ mg GAE/g) and, flavonoid content (۳۸.۵-۱۴۴.۱۳ mg RE/g) among different genotypes peel. The pomegranate peel is considered as an agro-waste but the present study provides evidence that the pomegranate peels are a potential source of natural antioxidant.

کلیدواژه ها:

Pomegranate Peel ، Phenolic compounds ، Ellagic acid ، Punicalagin ، Antioxidant capacity