The Effect of miR-۳۴a and miR-۳۴b Polymorphisms on Clinical-Pathological Characteristics of Renal Cell Carcinoma

Introduction: MicroRNAs can regulate expression of gene by binding to ۳'-UTRs (untranslated region) of mRNAs. Genetic changes in miRNA binding domains affect susceptibility to various diseases, including cancer. This research presented to determine the impact of pri-miR-۳۴b T> C rs۴۹۳۸۷۲۳ and miR۳۴a A>C rs۶۵۷۷۵۵۵ polymorphism in renal cell carcinoma.  Method: This case-control study has been done on ۱۰۰ patients with RCC and ۱۰۰ healthy matched control in two referral urology centers in Tehran, Iran. The miRNA-۳۴a/b polymorphisms mentioned in the genomes of healthy individuals and patients with RCC were studied by the tetra arms PCR method. Then statistical studies were performed by SPSS software, using the independent sample T-test and Chi-square. A P-value lower than ۰.۰۵ is significant.Results: The connection between pri-miR-۳۴b T>C and miR۳۴a A>C variants were considered according to age, sex, tumor site, metastasis site, tumor stage, and tumor grade in this study. No statistically significant difference existed between the two study groups regarding gender and age. Smoking and alcohol consumption were significantly higher in RCC patients, so we suggest them as the main risk factors. The rs۶۵۷۷۵۵۵ polymorphism showed a significant association with the classification of tumor type (P-value=۰.۰۴۷), but rs۴۹۳۸۷۲۳ SNP did not correlate with the tumor type.    Conclusions: The findings indicated that rs۴۹۳۸۷۲۳ and rs۶۵۷۷۵۵۵ polymorphism might be a risk for predisposition to RCC in the population of Iran. More research with greater sample sizes and various ethnicities must approve our findings.

کلیدواژه ها:

miRNAs ، risk factor ، cancer ، renal cell carcinoma