Cloning and expression of the Brucella melitensis OMP۲۸ and OMP۳۱ genes in Lactococcus Lactis and investigations of immunogenic responses in BALB/c Mice

BACKGROUND AND OBJECTIVESBrucellosis is a common disease between human and animals in the world and the disease imposes a huge costs on the economy and society. There is still no suitable vaccine against this disease. Currently, the Live Brucella vaccines have disadvantages such as the inability to differentiate infected animals from vaccinated with classical Brucella tests in the first year, antibiotic resistance, the possibility of shedding the vaccine strain from milk and other animal secretions and the possibility of abortion of vaccinated animals. Finally, the virulence potential of common vaccine strains for humans led to the evaluation of the OMP۳۱ and OMP۲۸ recombinant proteins and their immunogenicity in the prevention of the disease.MATERIALS AND METHODSIn this study, OMP۲۸ and OMP۳۱ recombinant proteins were isolated from Brucella melitensis and cloned in lactococcus lactis NZ۳۹۰۰ vector. Colonies that have received the vector will turn yellow on this medium. After transformation of the genes to the host and the expression of the recombinant protein, mice were given the bacteria as an oral vaccines. Evaluation of the immunogenicity of the vaccine was performed orally in mice (۲-۵ x ۱۰۹ cells for each mouse). Serum samples were collected from all mice on days ۰, ۷, ۱۴, ۲۱, ۲۸ and antibody levels were measured against OMP۲۸ by ELISA method.RESULTS AND DISCUSSIONThe results showed a significant increase in the antibody titer of the vaccinated groups against OMP۲۸ and OMP۳۱ compared to the control group. It should be noted that all rats were weighed before the test and quarantined for two weeks for environmental compatibility in the laboratory.CONCLUSIONAll these attempts to express this protein and investigate its antigenicity, indicating the high antigenitivity of protein derived from OMP۳۱ and OMP۲۸ gene expression.

کلیدواژه ها:

Expression ، OMP ، Cloning ، Brucella Melitensis ، Immunogenicity